How to Use Ozonated Glycerin: Nasal Spray, Drinking, Nebulizing and Skin

How to Use Ozonated Glycerin: Nasal Spray, Drinking, Nebulizing and Skin

Ozonated glycerin is usually diluted before use: about 10% in saline for a nasal spray, ½ teaspoon to 1 tablespoon a day in water to drink, 2.5% to 10% in distilled water to nebulize, and 10% in lotion for skin.

Glyzine™ is about 10,000 ppm, so a little goes a long way. This guide covers what to mix it with and how much to use, with a simple mixing table at the end.

This article is part of our Ozonated Glycerin (Glyzine™) guide series.

How Do You Use Ozonated Glycerin as a Nasal Spray?

Mix it with saline in a nasal pump bottle.

  • 10% strength
  • Start at 5% if you're sensitive
  • Never use tap water in your nose

How Do You Drink Ozonated Glycerin?

Mix it with water, or cooled tea or coffee. It tastes sweet. Work up slowly:

  • Week 1: ½ teaspoon a day
  • Week 2: 1 teaspoon a day
  • After that: up to 1 tablespoon a day
  • Too much at once can loosen stools. If that happens, take less.

How Do You Nebulize Ozonated Glycerin?

Mix it with distilled water. Never nebulize it undiluted.

  • Start at 2.5%, then step up to 5% and 10% after a few comfortable sessions
  • Sensitive? Start at 1%
  • About 1 teaspoon (5 mL) per session, until the cup is empty (10 to 15 minutes)
  • Once or twice a day
  • Use a plug-in compressor nebulizer with a mask. Mesh and ultrasonic units don't work well with glycerin.
  • Keep leftover mix in the fridge and use it within 24 hours

For equipment, a full mixing table and a step-by-step session, see how to nebulize ozonated glycerin.

How Do You Use Ozonated Glycerin on Skin?

Mix it with your lotion or moisturizer.

  • Everyday face and body: 10%
  • Spots: full strength, dabbed on 2 to 3 times a day
  • Scalp: a small amount in your palm, massaged in
  • Try a small patch of skin first

For a full routine, see how to use Glyzine™ in a skincare routine.

How Do You Use Ozonated Glycerin in Your Mouth?

Mix it with water.

  • Mouthwash: 10%, plus a drop of peppermint oil if you like. Swish for 1 to 2 minutes and spit.
  • Gums: full strength. Massage a pea-sized amount into the gum line before bed.

Can You Use Ozonated Glycerin on Pets?

Mix it with distilled water in a spray bottle.

  • Skin and coat: 5%. Spray it on and rub it in if you can.

How Do You Mix Ozonated Glycerin?

Start with 3 tablespoons of liquid, then add this much Glyzine™:

Add to 3 tbsp of liquid Strength Use it for
1 teaspoon 10% Nasal spray, mouthwash, skin
½ teaspoon 5% Pets, nebulizer step-up
¼ teaspoon 2.5% Nebulizer start

Mix it fresh. Keep leftovers in the fridge and use them within 24 hours.

Frequently Asked Questions

Why does ozonated glycerin need to be diluted?

Glyzine™ is about 10,000 ppm. That's strong enough to use full strength on small spots and gums, but sprays, mouthwash and nebulizers use a diluted mix.

Should I use distilled water or saline?

Use saline for your nose, distilled water for a nebulizer or spray bottle, and plain water, or cooled tea or coffee, for drinking. Never put tap water in your nose or nebulizer.

How much should I put in a nebulizer?

About 5 mL (1 teaspoon) of a 2.5% to 10% mix per session, once or twice a day.

How long does a mix last?

Use it within 24 hours and keep it in the fridge. Undiluted Glyzine™ lasts much longer; see how long ozonated glycerin lasts.

Where Do These Amounts Come From?

These amounts follow the Glyzine™ application guide and published guidance from ozone practitioners, including Dr. Robert Rowen's nebulizing guidelines, which start at 1% to 2.5% and allow up to 5 mL twice a day.

Get Glyzine™

Glyzine™ is pure ozonized glycerin, about 10,000 ppm, made from USP-grade organic vegetable glycerin in a cleanroom built to ISO Class 5 specifications. Each batch is made to order and ships within two business days of production.

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Written by Nattha Wannissorn, PhD

Dr. Wannissorn wrote the majority of the research-based articles in this Knowledge Center, translating peer-reviewed literature into plain language with sources cited inline.

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  • Nattha Wannissorn, PhD
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