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Ozonated Glycerin — glyzine

Ozonated glycerin is one of the newest formats in ozone skincare, and one of the least understood. This guide exists to fix that. It covers what ozonated glycerin is, how it differs chemically from ozonated oils, what the published research does and doesn't show, and how to use it in a daily routine.

We make Glyzine(TM), our ozonated glycerin, at a stated concentration of approximately 10,000 ppm from USP-grade organic vegetable glycerin in an ISO Class 5 cleanroom environment. It is, as far as we know, the first ozonated glycerin backed by an independent LC-MS chemical profile, and we publish what that analysis can and cannot tell us. The science on this ingredient is early - a small number of controlled cosmetic studies and a growing body of laboratory work - and these articles say so plainly.

Start with "What Is Ozonated Glycerin" if you're new, or the chemical profile if you want the chemistry first.
9 articles

PurO3 vs Simply O3 Ozonated Glycerin: A Side-by-Side Comparison

At 2 oz, Glyzine is $49.99 and PurO3 is $59.95, both shipped free. Glyzine adds a cleanroom built to ISO Class 5 standards, a published strength and published LC-MS data.

Micah Lowe · Updated October 2026

How to Use Ozonated Glycerin: Nasal Spray, Drinking, Nebulizing and Skin

How much ozonated glycerin to use and what to mix it with: about 10% in saline for a nasal spray, ½ teaspoon to 1 tablespoon a day to drink, 2.5% to 10% to nebulize, and 10% in ...

Nattha Wannissorn, PhD · Updated October 2026

How to Nebulize Ozonated Glycerin: Equipment, Mixing Table and Steps

Mix ozonated glycerin with distilled water (start at 2.5%), put about 5 mL in a plug-in compressor nebulizer with a mask, and breathe the mist for 10 to 15 minutes. Equipment, m...

Nattha Wannissorn, PhD · Updated October 2026

How Long Does Ozonated Glycerin Last?

Ozonated glycerin shelf life depends on storage temperature, container type, and how it was made. Here’s what the published research shows — and what it doesn’t.

Micah Lowe · Updated October 2026

Is Ozonated Glycerin Safe to Use?

Ozonated glycerin is well-tolerated by most people when made from high-purity ingredients and produced correctly. Here’s what the evidence shows — and what to watch for.

Nattha Wannissorn, PhD · Updated October 2026

Glyzine Skincare Routine: How to Use Ozonated Glycerin for Skin

Ozonated glycerin offers a unique approach to ozone skincare — water-soluble, odor-free, and non-greasy. Learn how to use Glyzine in your daily routine and what the emerging res...

Nattha Wannissorn, PhD · Updated October 2026

Ozonized Glycerin vs. Ozone Oil: What Are the Differences?

Ozonized glycerin and ozone oil are both ozone-infused products, but their chemistry, texture, and use cases are fundamentally different. Here's how to choose between them.

Nattha Wannissorn, PhD · Updated October 2026

What's Really in Ozonized Glycerin? | Chemical Profile

SimplyO3 shares what independent analysis revealed about the chemical composition of ozonized glycerin — including glyceric acid, glycolic acid, and why pure oxygen matters in p...

Nattha Wannissorn, PhD · Updated October 2026

What is Ozonated Glycerin (Glyzine)?

Ozonated glycerin combines ozone's oxidative chemistry with glycerin's natural humectant properties — resulting in a water-soluble, odor-free format for ozone wellness. Learn wh...

Nattha Wannissorn, PhD · Updated October 2026