How to Nebulize Ozonated Glycerin: Equipment, Mixing Table and Steps

How to Nebulize Ozonated Glycerin: Equipment, Mixing Table and Steps

To nebulize ozonated glycerin, mix it with distilled water (start at 2.5%), put about 5 mL in a plug-in compressor nebulizer with a mask, and breathe the mist normally for 10 to 15 minutes, once or twice a day.

Diluted in water and run through an ordinary compressor nebulizer, Glyzine™ ozonated glycerin becomes a fine mist you breathe in. This guide covers the equipment, how strong to make it, a mixing table and a step-by-step session.

This article is part of our Ozonated Glycerin (Glyzine™) guide series. For every other way to use it, see how to use ozonated glycerin.

What Do You Need to Nebulize Ozonated Glycerin?

A compressor nebulizer with a mask

Use a plug-in compressor nebulizer with a mask, not a mesh or battery handheld or an ultrasonic unit

Use a plug-in compressor nebulizer (also called a jet or "pump" nebulizer) that comes with a face mask. It's the standard tabletop kind with a small air pump, a tube, a liquid cup and a mask.

  • Compressor type. Don't use a mesh or ultrasonic nebulizer: glycerin clogs the mesh, and ultrasonic units are unreliable with it.
  • Plugs into the wall. Plug-in units make a denser mist than battery handhelds.
  • Face mask included. Many name-brand kits ship with only a mouthpiece, so check the box contents.

Any unit that plugs into the wall and lists an adult mask in the box works. Most run $50 to $100. See compressor nebulizers with masks on Amazon.

Measuring tools and water

1 mL oral syringe, 10 mL syringe, small glass cup and distilled water

Item What it's for Notes
1 mL oral syringe, no needle, marked in 0.01 mL Measuring the ozonated glycerin Glycerin is thick. Draw it slowly and wipe the tip before dispensing. Covidien Monoject 1 mL oral syringe
10 mL syringe, no needle Measuring the water SimplyO3 Ozone Syringe, 10cc
Small glass cup or shot glass Mixing Glass, not plastic
Distilled water The liquid Saline also works. Never use tap water.

Keep one syringe for the glycerin and one for water, and label them so they don't get swapped.

How Strong Should the Mix Be?

Strength is the percentage of ozonated glycerin in the total mix. Start light and increase only if it feels comfortable.

Strength steps: 1% extra gentle, 2.5% start here, 5% step up, 10% upper limit

  • 1%: extra gentle
  • 2.5%: start here
  • 5%: step up
  • 10%: upper limit

Move up one step after a few comfortable sessions. If a session feels heavy, go back to the previous strength.

How Do You Mix It?

Most nebulizer cups hold 5 to 8 mL. Mix a 5 mL batch for each session, or a 10 mL batch if you want easier measuring at the low strengths.

Strength For a 5 mL cup For a 10 mL batch
1% 0.05 mL glycerin + 4.95 mL water 0.1 mL glycerin + 9.9 mL water
2% 0.1 mL glycerin + 4.9 mL water 0.2 mL glycerin + 9.8 mL water
2.5% 0.125 mL glycerin + 4.875 mL water 0.25 mL glycerin + 9.75 mL water
5% 0.25 mL glycerin + 4.75 mL water 0.5 mL glycerin + 9.5 mL water
10% 0.5 mL glycerin + 4.5 mL water 1.0 mL glycerin + 9.0 mL water

Measuring 0.05 mL is fiddly. For 1%, mix the 10 mL batch, load 5 mL into the cup, and keep the rest in the fridge for your next session within 24 hours.

How Do You Do a Session?

  1. Step 1: Measure the ozonated glycerin with a 1 mL syringe into a glass cupMeasure the ozonated glycerin with the 1 mL syringe and put it in the glass cup.
  2. Step 2: Add water with a 10 mL syringeMeasure the water with the 10 mL syringe and add it.
  3. Step 3: Swirl for 2 to 3 minutesSwirl for 2 to 3 minutes. Glycerin doesn't want to mix with water, so keep swirling until the liquid looks even.
  4. Step 4: Pour into the nebulizer cup and attach it to the maskPour it into the nebulizer cup and attach the cup to the mask.
  5. Step 5: Connect the tube and turn the compressor onConnect the tube to the compressor and turn it on. You should see mist within a few seconds.
  6. Step 6: Put on the mask and breathe in gently through the nosePut on the mask and breathe normally, sitting upright and relaxed. Don't force deep breaths; breathe in gently through the nose.
  7. Step 7: Run until the cup is empty, 10 to 15 minutesRun until the cup is empty, about 10 to 15 minutes.
  8. Step 8: Rinse the cup and mask and let them air dryRinse the cup and mask with warm water and let them air dry. Replace the cup on the manufacturer's schedule, since glycerin residue builds up.

How Often Can You Nebulize It?

Once or twice a day, or whenever you want it.

Good to Know

  • If the mist feels heavy, stop and use a weaker mix next time.
  • Never nebulize undiluted ozonated glycerin. Always mix it with water first.
  • A mixed batch keeps in the fridge for up to 24 hours.

Frequently Asked Questions

How much ozonated glycerin do you put in a nebulizer?

About 5 mL of mix per session. At 2.5%, that's 0.125 mL of ozonated glycerin plus 4.875 mL of distilled water.

Can you use saline instead of distilled water?

Yes. Distilled water is preferred because it's easier to find, but saline works too. Never use tap water.

Why not a mesh or ultrasonic nebulizer?

Glycerin is thick enough to clog a mesh, and ultrasonic units don't mist it reliably. A plug-in compressor nebulizer handles it well.

Where Do These Amounts Come From?

These amounts follow published guidance from ozone practitioners, including Dr. Robert Rowen's nebulizing guidelines, which start at 1% to 2.5% and allow up to 5 mL twice a day.

Get Glyzine™

Glyzine™ is pure ozonized glycerin, about 10,000 ppm, made from USP-grade organic vegetable glycerin in a cleanroom built to ISO Class 5 specifications. Each batch is made to order and ships within two business days of production.

Continue Reading

Sources

This guide is product-use information only. Follow your nebulizer manufacturer's instructions. Stop if you feel any discomfort, and talk with a healthcare professional if you have questions about whether it is right for you.

Written by Nattha Wannissorn, PhD

Dr. Wannissorn wrote the majority of the research-based articles in this Knowledge Center, translating peer-reviewed literature into plain language with sources cited inline.

Meet the editorial team →

← Previous Post Next Post →

  • |
  • Nattha Wannissorn, PhD
Comments 0
Leave a comment
Your Name:*
Email Address:*
Message: *

Please note: comments must be approved before they are published.

* Required Fields