Why the amount and concentrations of ozone produced matter for high-grade use
When it comes to working with ozone at a high grade, precision matters, whether ozone is being used for lab experiments, sanitation, or other controlled applications, you want to know the exact amount of ozone being produced.
Ozone concentration, measured in micrograms per milliliter (µg/mL or mcg/mL), represents how much ozone is present in each milliliter of gas being delivered. This refers to the amount of ozone that comes right out of the machine. Since ozone is an unstable molecule, it decays into oxygen quickly, and you’ll start to lose ozone concentration within a few minutes.
Certain applications require particular ozone concentrations to be effective or to avoid damaging sensitive materials.
This article will make it straightforward to figure out the amounts of ozone produced.
How do SimplyO3 ozone generators adjust ozone concentration?
SimplyO3’s ozone generators, Stratus and Cumulus, produce ozone using cold corona discharge technology.
This method generates a stable stream of ozone gas by applying an electrical current to 99.9% pure oxygen as it passes through a sealed ozone cell.
It’s a reliable and widely used technique for creating high-purity ozone without the risk of heat degradation.
Each machine adjusts ozone concentration in a slightly different way.
Stratus
With the Stratus, the concentration is controlled entirely by the oxygen flow rate.
The flow rate, or the rate at which oxygen passes through the ozone generator, controls the ozone concentration. Faster oxygen flow dilutes the ozone, while slower flow produces a more concentrated output.
Lower flow rates mean the oxygen spends more time in the ozone chamber, allowing a stronger concentration of ozone to build up.
Higher flow rates reduce contact time and produce a lower ozone concentration.
This streamlined approach makes the Stratus a straightforward, easy-to-use option for those who want consistent performance with minimal setup.
You need our specialized regulator that finely adjusts the oxygen flow rate, which will allow you to produce the ozone concentrations corresponding to the chart. The higher number on the regulator corresponds to slower oxygen flow, but more concentrated ozone. If you’re filling a bag or a syringe, a higher flow setting number will take longer to fill a bag.
Whereas other oxygen regulators, such as ones used for oxygen inhalation, show higher numbers for faster oxygen flow.
The Stratus 3.0 Concentration Chart Explained
The left column on the chart is the flow setting on the regulator. The right column provides the output ozone concentration in micrograms per mL (µg/mL or mcg/mL). This concentration unit is the same as gamma, mg/L, and parts per million (ppm).
Step-by-step example calculations for syringe and bag fills.
Here are a few more examples of scenarios of calculating the amount of ozone produced.
The formula is:
Ozone Concentration (µg/mL) from the chart × Volume (mL) = Total Ozone in Micrograms (µg)
Example 1: Syringe fill
Scenario: You are filling a 60 mL syringe using the Stratus setting 7.
Step 1: Check your concentration
The chart shows your chosen setting yields 33 µg/mL.
Step 2: Confirm your volume
You will be filling the syringe with 60 mL of ozone-oxygen gas.
Step 3: Apply the formula: 33 µg/mL × 60 mL = 1,980 µg
Result: The 60 mL syringe contains 1,980 micrograms of ozone.
Example 2: Ozone bag fill
If you use the Stratus flow setting 4 and fill up to the first chamber (200 mL) of our ozone bag;
Step 1: Check your ozone concentration 18 µg/mL
Step 2: Confirm your volume
You will be filling the bag with 200 mL of ozone-oxygen gas.
Step 3: Apply the formula: 18 µg/mL × 200 mL = 3,600 µg
Result: The ozone bag contains 3,600 micrograms of ozone.
Cumulus
Cumulus allows for finer adjustments and a wider range of concentration settings, which may be necessary for some professional use.
Cumulus Concentration Chart Explained
The Cumulus is engineered for users who require finer control over ozone concentration.
The specialized regulators for the Cumulus have 0, 1/16, ⅛, ¼, 1/2, ¾, and 1 LPM flow settings. They’re not the same as the regulators for the Stratus. Lowering the flow rate while increasing the setting increases the concentration.
Besides the oxygen flow rate, you can adjust the electricity in the corona discharge with the power dial on the machine to determine the final output concentration.
This dial enables you to fine-tune the ozone dosage with much greater precision, making the Cumulus an ideal choice for various professional applications.
The Cumulus Concentration Chart shows ozone concentration (µg/mL) based on the combination of:
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Flow Rate: Measured in LPM on the leftmost column.
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Generator Setting: A 1–10 scale on the device, shown along the top row.
Each cell on the chart represents the ozone concentration produced when a specific flow rate and generator setting are used together.
For example, a flow rate of 1/2 LPM at setting 5 will yield a concentration of 17 µg/mL.
How to Calculate the Total Amount of Ozone Produced with the Cumulus:
The Cumulus concentration chart with multiple columns (1 - 10) has more concentrations to choose from. This is helpful for professionals who are titrating in more specific ozone doses.
Example 1: Ozone syringe fill example 1 - 56 µg/mL in two syringes, making it 120 mL
Step 1: Decide what concentration you want.
Here, you want to make ozone gas at 56 µg/mL in 120 mL of volume.
Step 2: Find the Generator Setting and LPM value in the chart.
See the number 56 highlighted in the image below.
You will notice above this number is setting number “4”.
To the left-hand column is “1/8”.
Now that we found the concentration we want, set the regulator on the oxygen tank to 1/8. Next, set the dial to the machine Ozone Generator to 4.
Once we turn the ozone generator on, it will be dispensing ozone at 56 ug/ml (gamma).
Ozone Bag Fill Example 2
Scenario: You are filling an ozone bag to 400 mL using the Cumulus at generator setting 5 and flow setting ¼ LPM.
Step 1: Check your concentration
The chart shows your chosen setting yields 33 µg/mL.
Step 2: Confirm your volume
You will be filling the bag with 400 mL of ozone-oxygen gas.
Step 3: Multiply: 33 µg/mL × 400 mL = 14,800 µg
Result: The ozone bag contains 14,800 micrograms of ozone.
Conclusion
In some cases, the total amount of exposed ozone, whether over a single occasion or over time, matters. The formula concentration x volume helps you calculate the total ozone you’re producing in micrograms.
